Phasor-FLIM and SHG imaging for quantitative analysis of lung cancer autofluorescence.

ABSTRACT: Histopathology using hematoxylin and eosin (H&E) staining remains the gold standard for tumor diagnosis. However, extracting quantitative data from stained slides is challenging, limiting the ability to obtain objective biomarkers for disease progression. Tissue autofluorescence provides an alternative by exploiting endogenous fluorophores, such as collagen, elastin, and NAD(P)H, which provide optical signatures of tissue pathology. Fluorescence Lifetime Imaging Microscopy (FLIM), when combined with phasor-based analysis, enables quantitative, fit-free assessment of metabolic and structural changes in tissues, simplifying data interpretation and enhancing diagnostic accuracy. In this study, we applied a phasor-FLIM approach to systematically analyze two distinct histotypes of non-small cel

SUBMITTER: Pesce L 

PROVIDER: S-EPMC12392685 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature