Spatial Molecular Heterogeneity on Biofunctionalized Particles Quantified by Three-Dimensional Single-Molecule DNA-PAINT.

ABSTRACT: Quantifying and controlling the spatial molecular heterogeneity on biofunctionalized particles is essential for understanding and improving their functionality in bioscience applications. Here, we describe an analysis framework based on single-molecule localization microscopy that can quantitatively assess the spatial molecular properties of affinity molecules conjugated to particles. We performed 3D DNA-PAINT imaging on biofunctionalized particles and established analysis methods to correlate single-molecule data to the particle outer surfaces, count the number of conjugated molecules, and quantify the spatial distributions of the conjugated molecules. We show that imaging data combined with simulation-based molecular counting gives access to high densities of conjugated molecules and ena

SUBMITTER: Tan WS 

PROVIDER: S-EPMC12392723 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature