Expanding Targeted Instrumentation for Discovery Applications: Complement Reporter Ion Quantification with a Quadrupole-Ion Trap Instrument.

ABSTRACT: Proteomic workflows have traditionally been divided into discovery-based and targeted approaches with instrumentation optimized specifically for each. Discovery experiments typically utilize high-resolution analyzers, while targeted workflows rely on the sensitivity and specificity of triple quadrupole systems. Recently, a quadrupole-ion trap instrument (Stellar MS) has demonstrated superior performance for targeted applications compared to conventional triple quadrupoles. In this study, we expand the capabilities of this platform to multiplexed shotgun proteomics using complement reporter ion quantification in an ion trap (iTMTproC). Benchmarking experiments with defined standards show that iTMTproC achieves quantification accuracy and interference reduction comparable to MultiNotch MS

SUBMITTER: Cruz ER 

PROVIDER: S-EPMC12392913 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature