Advanced neural activity mapping in brain organoids via field potential imaging with ultra-high-density CMOS microelectrode arrays.

ABSTRACT:

Introduction

Human iPSC-derived brain organoids and assembloids have emerged as promising in vitro models for recapitulating human brain development, neurological disorders, and drug responses. However, detailed analysis of their electrophysiological properties requires advanced measurement techniques.

Methods

Here, we present an analytical approach using ultra-high-density (UHD) CMOS microelectrode arrays (MEAs) with 236,880 electrodes across a 32.45 mm2 sensing area, enabling large-scale field potential imaging (FPI) of brain organoids.

Results

Neuronal activity was recorded from over 46,000 electrodes, allowing single-cell spike detection and network connectivity analysis. In midbrain organoids, L-DOPA administration elicited both excitatory and inhibitory response

SUBMITTER: Yokoi R 

PROVIDER: S-EPMC12392918 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature