A module with multiple transcription factors positively regulates powdery mildew resistance in grapevine.

ABSTRACT: Erysiphe necator, the causal agent of powdery mildew (PM), is a fungal pathogen that can severely reduce crop yields. In this study, stable overexpression and silencing demonstrated that the transcription factor VqWRKY46 was identified as a positive regulator of PM resistance in grapevine. This enhanced resistance was associated with increased callose deposition and hypersensitive response cell death. We also demonstrated that VqLIMYB specifically activated VqWRKY46 expression by binding to a CAGTTA motif in its promoter. Moreover, VqWRKY46 interacted with VqNF-YC9 and formed homodimers, positively regulating VqNF-YC9 expression by directly binding to three W-box motifs in its promoter. Additionally, DAP-Seq analysis identified VqDSC1, a TIR-NB-LRR resistance protein, as a downstream targe

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12392958 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature