Morphology effect of a novel biocompatible nucleic acid delivery nanosystem of g-C<sub>3</sub>N<sub>4</sub>@dsRNA for application in plant gene expression and plant virus disease protection.
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ABSTRACT: Delivery of exogenous nucleic acid to intact plants is a desirable but challenging technology due to the dominant transport barrier posed by the plant cell wall. Here, we found that three different morphologies of g-C3N4 nanomaterials synthesized from urea can assist in the delivery of exogenous nucleic acids into mature leaf cells of Nicotiana benthamiana. Among these, g-C3N4 carbon dots (CDs) showed a higher ability to deliver exogenous nucleic acid compared to nanoporous and g-C3N4 nanosheets. The delivery ability of exogenous dsRNA and plasmid DNA by g-C3N4 could also be restrained by stomatal closure and the endocytosis pathways in plant cells. Furthermore, the coupling of g-C3N4
SUBMITTER: Wei X
PROVIDER: S-EPMC12392969 | biostudies-literature | 2025 Sep
REPOSITORIES: biostudies-literature
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