A chaperonin BnaC01.CCT8 contributes to silique length and seed weight by affecting auxin and jasmonic acid signalling in Brassica napus.

ABSTRACT: Seed weight (SW), which is directly influenced by silique length (SL), is a critical agronomic trait significantly affecting both the quality and yield of rapeseed. In this study, a shorter silique length (ssl) mutant was generated through ethyl methane sulfonate mutagenesis, exhibiting reduced SL and SW compared to the wild type. Utilizing a map-based cloning approach, BnaC01.CCT8, a member of the chaperonin containing T-complex polypeptide-1 (CCT) family, was identified as the gene responsible for restoring the ssl phenotype. A missense mutation from alanine to valine (A507V) in BnaC01.CCT8 was identified as crucial for its functional activity, as evidenced by the genetic complementation of BnaC01.CCT8 and BnaC01.CCT8^A507V in the Arabidopsis cct8-2 background. Moreover, overex

SUBMITTER: Qu Z 

PROVIDER: S-EPMC12392976 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature