Studies of Isonitrile Synthases Help Elucidate the Mechanism of Isonitrile Installation.

ABSTRACT: Isonitrile functional group is abundant in natural products and associated with various biological properties. To date, two pathways are known for isonitrile group installation. One is catalyzed by mononuclear nonheme iron dependent isonitrilases in which the reaction mechanism involves consecutive desaturation and decarboxylation processes. The other pathway includes isonitrile synthases, however, how isonitrile synthases enable the condensation of a sugar phosphate and an aromatic amino acid to install isonitrile group remains understudied. In this study, we examine substrate analogs and quantify reaction products using isotopologues to establish the substrate requirements of two isonitrile synthases, WelI1 and PIsnA, providing insights into their spatial and substrate requirements. Unde

SUBMITTER: Chen TY 

PROVIDER: S-EPMC12393152 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature