Impact of tissue sample preparation methods on myelin-sensitive quantitative MR imaging.

ABSTRACT:

Purpose

Validation of quantitative MRI (qMRI) parameters with histology is often done with ex vivo fixed tissue samples. Freezing is another common form of tissue preservation, but the effects of freezing and thawing tissue on myelin-sensitive quantitative MRI parameters and their correlation with histology require further analysis.

Methods

Myelin water imaging, off-resonance RF saturation magnetization transfer (MT), and selective inversion recovery MT MRI experiments were conducted on 14 fresh, thawed, and fixed sheep brain tissue samples to calculate various surrogate measures of myelin content. These measures were compared with luxol fast blue (LFB) histological stain results.

Results

Fresh, thawed, and fixed tissue qMRI values correlated well with LFB. Thawed and

SUBMITTER: Murguia A 

PROVIDER: S-EPMC12393208 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature