FAIR sharing of Chromatin Tracing datasets using the newly developed 4DN FISH Omics Format.

ABSTRACT: A key output of the NIH-Common Fund 4D Nucleome (4DN) project ^1,2 is the open publication of datasets related to the structure of the human cell nucleus and the genome. Recent years have seen a rapid expansion of multiplexed Fluorescence In Situ Hybridization (FISH) or FISH-omics methods, which quantify the spatial organization of chromatin in single cells, sometimes together with RNA and protein measurements, and provide an expanded understanding of how 3D higher-order chromosome structure relates to transcriptional activity and cell development in both health and disease. Despite this progress, results from Chromatin Tracing FISH-omics experiments are difficult to share, reuse, and subject to third-party downstream analysis due to the lack of standard specifications for data e

SUBMITTER: Navelkar R 

PROVIDER: S-EPMC12393234 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature