Active regulation of the epidermal growth factor receptor by the membrane bilayer.

ABSTRACT: Cell surface receptors transmit information across the plasma membrane to connect the extracellular environment to intracellular function. While the structures and interactions of the receptors have been long established as mediators of signaling, increasing evidence suggests that the membrane itself plays an active role in both suppressing and enhancing signaling. Identifying and investigating this contribution has been challenging owing to the complex composition of the plasma membrane. We used cell-free expression to incorporate the epidermal growth factor receptor (EGFR) into nanodiscs with defined membrane compositions and characterized ligand-induced transmembrane conformational response and interactions with signaling partners using single-molecule and ensemble fluorescence assays.

SUBMITTER: Srinivasan S 

PROVIDER: S-EPMC12393268 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature