Spike mutations that affect the function and antigenicity of recent KP.3.1.1-like SARS-CoV-2 variants.

ABSTRACT: SARS-CoV-2 is under strong evolutionary selection to acquire mutations in its spike protein that reduce neutralization by human polyclonal antibodies. Here we use pseudovirus-based deep mutational scanning to measure how mutations to the spike from the recent KP.3.1.1 SARS-CoV-2 strain affect cell entry, binding to ACE2 receptor, RBD up/down motion, and neutralization by human sera and clinically relevant antibodies. The spike mutations that most affect serum antibody neutralization sometimes differ between sera collected before versus after recent vaccination or infection, indicating these exposures shift the neutralization immunodominance hierarchy. The sites where mutations cause the greatest reduction in neutralization by post-vaccination or infection sera include receptor-binding doma

SUBMITTER: Dadonaite B 

PROVIDER: S-EPMC12393327 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature