Screening channelrhodopsins using robotic intracellular electrophysiology and single cell sequencing.

ABSTRACT:

Background

Our ability to engineer opsins is limited by an incomplete understanding of how sequence variations influence function. The vastness of opsin sequence space makes systematic exploration difficult.

New method

In recognition of the need for datasets linking opsin genetic sequence to function, we pursued a novel method for screening channel-rhodopsins to obtain these datasets. In this method, we integrate advances in robotic intracellular electrophysiology (Patch) to measure optogenetic properties (Excite), harvest individual cells of interest (Pick) and subsequently sequence them (Sequence), thus tying sequence to function.

Results

We used this method to sequence more than 50 cells with associated functional characterization. We further demonstrate the utilit

SUBMITTER: Ehrlich S 

PROVIDER: S-EPMC12393332 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature