RAB5 NUCLEOTIDE BINDING PROMOTES β-OXIDATION TO FUEL HEPATOCELLULAR CARCINOMA CELL PROLIFERATION.

ABSTRACT: Altered lipid metabolism and lipid droplet (LD) dynamics are hallmark features of hepatocellular carcinoma (HCC) subtypes, but the molecular mechanisms governing LD trafficking and catabolism in HCC cells remain unclear. The small GTPase Rab5, a key regulator of early endosomal dynamics, has been observed to localize to the surface of LDs, suggesting it may play a role in LD turnover. However, the regulation of Rab5-LD interactions and its functional consequences in HCC cell metabolism and proliferation have not been elucidated. In this study, we explored the role of Rab5 in governing LD homeostasis and its impact on HCC cell proliferation. We found that the GTP-bound (Q79L), active form of Rab5 exhibited increased association with LDs compared to the GDP-bound, inactive mutant (S34N). Nut

SUBMITTER: Otakhor KO 

PROVIDER: S-EPMC12393351 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature