Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events.

ABSTRACT: Enhancers serve as the major genomic elements regulating mammalian signal-dependent transcriptional programs, characterized by alternating periods of target gene "bursting" and "non-busting" that require investigation of induced enhancer condensates and locus motility in real time to provide dynamic insights into signal/ligand-dependent regulatory events. Here, endogenous live cell imaging has revealed the altered chromosomal dynamics/condensate formation occurring during estrogen receptor α (ERα)-dependent target gene bursting/post-bursting and chronic activation events. Simultaneous DNA/RNA endogenous live imaging reveals that an increased mobility of acutely ERα-stimulated loci observed during the bursting phase is, unexpectedly, further increased in the subsequent non-burst phase. Sing

SUBMITTER: Wang S 

PROVIDER: S-EPMC12393356 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature