Imaging cellular activity simultaneously across all organs of a vertebrate reveals body-wide circuits.

ABSTRACT: All cells in an animal collectively ensure, moment-to-moment, the survival of the whole organism in the face of environmental stressors^1,2. Physiology seeks to elucidate the intricate network of interactions that sustain life, which often span multiple organs, cell types, and timescales, but a major challenge lies in the inability to simultaneously record time-varying cellular activity throughout the entire body. We developed WHOLISTIC (WHole Organism Live Imaging System for recording Tissue and IntraCellular activity), a method to image second-timescale, time-varying intracellular dynamics across cell-types of the vertebrate body. By advancing and integrating volumetric fluorescence microscopy, machine learning, and pancellular transgenic expression of calcium sensors in transp

SUBMITTER: Ruetten VMS 

PROVIDER: S-EPMC12393375 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature