A Toolkit for <i>In Vivo</i> Mapping and Modulating Neurotransmission at Single-Cell Resolution.

ABSTRACT: Understanding the organization and regulation of neurotransmission at the level of individual neurons and synapses requires tools that can track and manipulate transmitter-specific vesicles in vivo. Here, we present a suite of genetic tools in Caenorhabditis elegans to fluorescently label and conditionally ablate the vesicular transporters for glutamate, GABA, acetylcholine, and monoamines. Using a structure-guided approach informed by protein topology and evolutionary conservation, we engineered endogenously tagged versions for each transporter that maintain their physiological function while allowing for cell-specific, bright, and stable visualization. We also developed conditional knockout strains that enable targeted disruption of neurotransmitter synthesis or packaging i

SUBMITTER: Cuentas-Condori A 

PROVIDER: S-EPMC12393400 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature