Automated chromatin profiling with spa-ChIP-seq uncovers the impacts of condition variations.

ABSTRACT: Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to study the genomic localization of DNA-associated proteins. However, conventional protocols include multiple manual steps that can introduce inconsistency and limit scalability, thereby restricting the inclusion of appropriate replicates and controls. Although the introduction of liquid handling platforms has improved reproducibility, most existing efforts have automated only a subset of the workflow, and extending automation to efficiently map non-histone proteins, such as chromatin regulators, remains challenging. Here, we present a fully automated implementation of our previously developed single-pot ChIP-seq protocol (Texari et al. 2021), named spa-ChIP-seq, which enables scalable processing of 8 to 96 ChI

SUBMITTER: Cao Y 

PROVIDER: S-EPMC12393402 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature