Spatiotemporal dynamics of signal dependent exocytosis and parasitophorous vacuolar membrane rupture during <i>Plasmodium falciparum</i> egress.

ABSTRACT: Malaria, caused by intracellular Plasmodium parasites, remains a major global health concern. These parasites reside and replicate within a vacuole in host red blood cells. Egress of daughter parasites out of the vacuolar and host membranes is tightly regulated via a complex mechanism. Prior studies have suggested that a cyclic-GMP driven calcium signaling pathway leads to the signal-dependent exocytosis of egress-specific vesicles that discharge several proteases into the parasitophorous vacuole. However, signal-dependent exocytosis during egress has not yet been observed in live parasitized RBCs. We targeted the exocytosis reporter, superecliptic pHlourin or SEP, to these egress-specific vesicles and utilized time-lapse imaging to observe exocytosis in live P. falciparum pa

SUBMITTER: Dedkhad W 

PROVIDER: S-EPMC12393438 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature