Characterization of membrane structures regulating primary ciliogenesis by quantitative isotropic ultrastructure imaging.

ABSTRACT: The trafficking, docking, and fusion of membrane vesicles at the mother centriole (MC) are required to construct the primary cilium. Here, we determined the three-dimensional (3D) membrane ultrastructures, and associated proteins, involved in primary cilium assembly upstream of axoneme growth. Our work reveals that the enlargement of small vesicles docked to the MC is a key trigger for ciliogenesis progression, a process requiring the MC distal appendage protein CEP164. We show these vesicles subsequently fuse to form tubular C-shaped and an unprecedented toroidal membrane intermediates, which ultimately organize into the ciliary vesicle covering the MC distal end. The formation of these previously uncharacterized tubular membrane ciliogenesis intermediates is orchestrated by the membrane

SUBMITTER: Lu Q 

PROVIDER: S-EPMC12393539 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature