Toggling of NKG2A expression drives functional specialization of iPSC-derived CAR NK cells.

ABSTRACT: Induced pluripotent stem cell (iPSC)-derived natural killer (iNK) cells offer a promising platform for off-the-shelf immunotherapy against hematological malignancies. NK cell function is dynamically regulated through education driven by inhibitory receptors, including CD94/NKG2A and killer cell immunoglobulin-like receptors (KIR). However, the acquisition of inhibitory receptors in iNK cells and their role during differentiation and education remains poorly defined. In this study, we monitored receptor repertoires, transcriptional states, and functional responses in a range of genetically engineered iNK cell lines. Transcriptional reference mapping placed iNK cells close to cytokine-activated NKG2A⁺ CD56^dim peripheral blood (PB) NK cells. Despite their early different

SUBMITTER: Kanaya M 

PROVIDER: S-EPMC12393553 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature