Comparison of iSeq and Miseq in 16S rRNA sequencing-based human gut microbiome analysis.

ABSTRACT: Illumina's MiSeq platform is a common approach in 16S-based microbiome analysis. Such usage is self-perpetuating in that many studies seek to employ widely used approaches to facilitate comparison of their results to existing literature. Yet, a range of factors, including cost and equipment availability can necessitate alternate approaches. For example, use of a Nano kit, lowers reagent costs by over 60% while others may only have access to entry-level sequencers such as Illumina's iSeq. The extent to which these approaches would impact results and subsequently conclusions is unknown. Attempting to address this question from the literature is complicated in that various studies not only use distinct cohorts but also differ in the reagents/methodologies to used to isolate DNA and generate s

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12393557 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature