DNA deformability defines sequence-dependent capture of <i>E. coli</i> gyrase.

ABSTRACT: Bacterial gyrase, unique among type II topoisomerases, introduces negative supercoils into DNA. Mechanistic details of gyrase still must be elucidated because of the complexity of the process and the difficulty in visualizing it. Specifically, the interplay among base sequence, local DNA deformability, and global DNA topology for gyrase site selection is unclear. To understand how gyrase interacts with DNA and selects a site of action, we created an ad hoc shape-based recognition methodology to ascertain DNA sequence from cryogenic electron microscopy densities as a string of purines and pyrimidines, which we conclusively matched to the DNA in our two previous structures of negatively supercoiled DNA bound to E. coli gyrase. The DNA helices to be cleaved by gyrase (the Gate o

SUBMITTER: Baker ML 

PROVIDER: S-EPMC12393610 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature