Human tonsil organoids reveal innate pathways modulating humoral and cellular responses to ChAdOx1.

ABSTRACT: The COVID-19 pandemic response demonstrated the effectiveness of adenovirus vector vaccines in inducing protective cellular and antibody responses. However, we still lack mechanistic understanding of the factors regulating immunity induced by this platform, especially innate pathways. We utilized a human tonsil organoid model to study the regulation of adaptive responses to ChAdOx1 nCoV-19. Innate activation and cytokine release occurred within 24 hours and T and B cell activation and antigen-specific antibody secretion occurred during the ensuing 14-day culture. Among the immune cell populations, plasmacytoid dendritic cells (pDCs) exhibited the highest ChAdOx1 transduction levels. pDC-derived IFN-ɑ was critical for humoral responses, but production of antigen in pDCs was dispensable. Fur

SUBMITTER: Pudjohartono MF 

PROVIDER: S-EPMC12393735 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature