Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis.

ABSTRACT:

Objective

This study aimed to investigate whether heliox preconditioning (HePC) alleviates neonatal acute respiratory distress syndrome (ARDS) by inhibiting oxidative stress and apoptosis, and to explore its potential mechanism.

Methods

Blood samples and bronchoalveolar lavage fluid (BALF) were collected from rat pups were randomly divided into control group, sham group, ARDS group, ARDS + CaMKII^- group, ARDS + CaMKII⁺ group, and ARDS + HePC group. We also investigated the role of CaMKII by manipulating its expression in vitro. Inflammatory markers, oxidative stress, apoptosis and activation of signaling pathways were assessed using histological staining, ELISA, Western blotting, qRT-PCR, Ca²⁺, immunofluorescence staining, and flow cyt

SUBMITTER: Ma J 

PROVIDER: S-EPMC12394224 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature