Tracking the infection dynamics of <i>Fusarium oxysporum</i> in <i>Codonopsis pilosula</i> based on GFP labelling.

ABSTRACT:

Introduction

Codonopsis pilosula root rot, caused by Fusarium oxysporum, has caused severe damage to the C. pilosula industry. Due to the unclear pathogenic mechanisms of F. oxysporum on C. pilosula, the effective implementation of control measures has been greatly restricted.

Methods

An efficient protoplast preparation and genetic transformation system was established for F. oxysporum FO-1, enabling real-time tracking of fungal colonization in C. pilosula. Single-factor experiments were conducted to determine optimal conditions, followed by response surface methodology to further optimize enzymatic parameters. PEG-mediated transformation was performed to generate GFP-tagged strains for infection tracking.

Results

Singl

SUBMITTER: Shi M 

PROVIDER: S-EPMC12394472 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature