3D Culture Analysis of Cancer Cell Adherence to <i>Ex Vivo</i> Lung Microexplants.

ABSTRACT: Ex vivo 3D culture of human tissue explants addresses many limitations of traditional monolayer cell culture techniques, namely the lack of cellular heterogeneity and absence of 3D intercellular spatial relationships, but presents challenges with regard to repeatability owing to the difficulty of acquiring multiple tissue samples from the same donor. In this study, we used a cryopreserved bank of human lung microexplants, ∼1 mm³ fragments of peripheral lung from donors undergoing lung resection surgery, and a liquid-like solid 3D culture matrix to describe a method for the analysis of non-small-cell lung cancer adhesion to human lung tissue. H226 (squamous cell carcinoma), H441 (lung adenocarcinoma), and H460 (large cell carcinoma) cell lines were cocultured with lung mic

SUBMITTER: Diodati NG 

PROVIDER: S-EPMC12394800 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature