Differential melting voltage by tandem-trapped ion mobility spectrometry: glycan structure influences glycoprotein stability.

ABSTRACT: Profiling the full spectrum of protein glycoforms is critical to understanding their functional roles. We developed the differential melting voltage approach using tandem-ion mobility/tandem-mass spectrometry and applied it to study Ribonuclease B glycoforms. Our results indicate that, in addition to glycan mass and intact protein size, the glycan structure plays a role in regulating the stability of Ribonuclease B.

SUBMITTER: Chai M 

PROVIDER: S-EPMC12394896 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature