Cooperative effects in DNA-functionalized polymeric nanoparticles.

ABSTRACT: DNA-functionalized nanoparticles (NPs), called spherical nucleic acids (SNAs), have attracted considerable attention due to their unique properties and numerous applications. In particular, DNA-functionalized dye-loaded polymeric NPs (DNA-NPs), owing to their exceptional fluorescence brightness, have emerged as powerful nanomaterials for the ultrasensitive detection and imaging of nucleic acids. Herein, we addressed a fundamental question unexplored for polymeric DNA-NPs: how does the dense packing of oligonucleotides on the particle surface impact their capacity to specifically hybridize with complementary sequences? Using Förster resonance energy transfer (FRET) between DNA-NPs and labelled complementary strands, we found that the DNA on the surface of the NPs exhibits dramatic enhanceme

SUBMITTER: Gaki P 

PROVIDER: S-EPMC12394930 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature