Enrichment and functional characterization of copper-binding peptides from food hydrolysates.

ABSTRACT: Imbalances in cellular copper are increasingly implicated in metabolic disorders. Food-derived peptides are gaining attention for their ability to alleviate metabolic disease symptoms with little to no toxicity. In this work, we enriched copper-binding peptides from enzymatic digestions of rice bran protein hydrolysates via Cu(ii)-based immobilized-metal affinity-based separations, identified the sequences by mass spectrometry, and performed physicochemical and sequence analysis of the enriched peptides. A subset of the enriched peptides representing a range of sequences and hydrophobicities were synthesized and individually assessed further for their solution-based Cu(ii) reactivity and cellular activity. Among the peptides tested, Cu(ii)-binding was most potent with those containi

SUBMITTER: Fernandez RL 

PROVIDER: S-EPMC12394934 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature