Optimization of the scale-up production process for high-yield laccase from white-rot fungi.

ABSTRACT:

Introduction

Laccase exhibits significant applications in food additives, wastewater treatment, and biomass processing. Fungal laccase exhibits high activity, good stability, and excellent performance. However, scale-up production of high-yield laccase from fungi is challenging. This study aimed to identify crucial factors affecting enzyme production and analyze the enzymatic properties of laccase during fermentation.

Methods

A laccase-producing white-rot fungus was used for fermentation process optimization in 200 L and 1200 L fermenters. The Plackett-Burman design revealed three significant influencing factors: temperature, aeration ratio, and agitation speed. The steepest ascent experiment was used to approximate the maximum response region, followed by the establishment

SUBMITTER: Ma Y 

PROVIDER: S-EPMC12394975 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature