The osteosarcoma immune microenvironment in progression: PLEK as a prognostic biomarker and therapeutic target.

ABSTRACT:

Introduction

Osteosarcoma (OS) is a malignant bone tumor with high metastatic potential and poor long-term survival. The tumor immune microenvironment and metabolic reprogramming are increasingly recognized as key drivers of OS progression, yet the molecular links between these systems remain unclear. This study aimed to identify immune-metabolic biomarkers in OS, focusing on pleckstrin (PLEK) as a potential regulatory hub.

Methods

We conducted differential expression and survival analyses using OS transcriptomic datasets and TCGA/GTEx data. Protein-protein interaction networks, GO/KEGG enrichment, and CytoHubba algorithms identified core hub genes. Tumor-infiltrating immune cells were profiled via TIMER. Single-cell RNA-seq (GSE162454) was used for immune and metabolic land

SUBMITTER: Zou Y 

PROVIDER: S-EPMC12394983 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature