Identification of a candidate rice blast resistance gene, <i>Pior4</i>(t), in an introgression line of <i>Oryza rufipogon</i> using CRISPR/Cas9-mediated genome editing.

ABSTRACT: Resistance breeding for rice blast is an economic strategy for protecting rice crops against this disease. Genes with nucleotide-binding site leucine-rich repeat (NBS-LRR) structures are known to contribute to disease resistance. Here, we identified a candidate resistance gene, named Pior4(t), associated with leaf and panicle blasts in an introgression line carrying the chromosome 4 segment of wild rice (Oryza rufipogon Griff.) backcrossed with the cultivar 'Nipponbare' (Oryza sativa L.). Mapping analysis based on leaf blast severity confirmed that Pior4(t) was localized in the 177-kb NBS-LRR cluster region. To identify the Pior4(t) sequence, mutant lines were generated by knocking out a candidate NBS-LRR gene in a homozygous line carrying Pior4(t)

SUBMITTER: Hayashi K 

PROVIDER: S-EPMC12395198 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature