Conserved mycobacterial sRNA B11 regulates lipooligosaccharide synthesis at posttranscriptional level in <i>Mycobacterium marinum</i>.

ABSTRACT: Extractable glycolipids of mycobacteria, such as lipooligosaccharides (LOSs), play crucial roles in responding to environmental stress and modulating the host immune response. Although the biosynthesis of LOS is likely regulated at multiple levels to ensure proper composition of the cell wall, the key regulators remain unknown. In this study, we investigated B11, a conserved mycobacterial small RNA (sRNA), and found that it post-transcriptionally regulates LOS synthesis in Mycobacterium marinum. Through a combination of RNA-seq and mass spectrometry screening, we identified specific genes within the LOS synthesis locus that are directly regulated by B11. We confirmed in vivo sRNA-mRNA interactions using MS2-tagged RNA affinity purification, and found that B11 utilizes the cytosine-r

SUBMITTER: Wang C 

PROVIDER: S-EPMC12395582 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature