Antimicrobial peptide PK34 modification enhances the antibacterial and anti-inflammatory effects of bone-derived mesenchymal stem cells in Mycobacterium tuberculosis infection.

ABSTRACT:

Background

New therapeutic strategies are needed to treat tuberculosis (TB). The antimicrobial peptide PK34 has a good ability to clear Mycobacterium tuberculosis (Mtb) and is not prone to drug resistance and adverse reactions. Mesenchymal stem cells (MSCs) can also be used as an adjunctive therapy for the treatment of TB. However, there have been no studies combining the two for the treatment of Mtb infection.

Methods

We aimed to construct bone-derived mesenchymal stem cells secreting the antimicrobial peptide PK34 (named Plent-PK34-BMSCs) and to investigate their roles in both in vitro and in vivo Mtb H37Rv infection models.

Results

We successfully constructed Plent-PK34-BMSCs that secrete and express the antimicrobial peptide PK34, and demonstrated that PK34 modifi

SUBMITTER: He XY 

PROVIDER: S-EPMC12395748 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature