Bidirectional shifts in Pm20d1 expression impact thermogenesis and metabolism.

ABSTRACT:

Background

Peptidase M20 domain containing 1 (PM20D1) is a secreted N-fatty acyl amino synthase and hydrolase that controls tissue and blood levels of N-fatty acyl amino acids. In brown adipocytes, N-fatty acyl amino acids bind to mitochondria and act as uncouplers of mitochondria, independent of UCP1. Interventions aimed at increasing or inhibiting PM20D1 expression considerably impact energy balance and metabolism; however, little is known about naturally occurring variants of the PM20D1/Pm20d1 gene and their impact on phenotype.

Methods

In vivo, gene expression of Pm20d1 in BALB/c, C57BL/6, and Ucp1 KO in brown adipose tissue and other metabolic tissues was measured. In vitro, transcriptional activity of Pm20d1 and brown adipocytes' oxygen consumption in primary culture w

SUBMITTER: Simoes MR 

PROVIDER: S-EPMC12395774 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature