P300 Promotes RUNX1 Transcription to Regulate Autophagy and Drive Corneal Neovascularization.

ABSTRACT: This study aimed to elucidate the regulatory role of Runt-related transcription factor 1 (RUNX1) in corneal neovascularization (CoNV) and its possible mechanisms. In VEGF-induced human umbilical vein endothelial cells (HUVECs), lentiviral vectors were used to knock down or overexpress RUNX1, and plasmid transfection was employed to knock down P300. EdU assay, Transwell assay, and tube formation assay were conducted to assess cell proliferation, migration, and tube formation ability, respectively. Chromatin immunoprecipitation, followed by quantitative PCR, was performed to verify the binding of P300 and RUNX1. Co-immunoprecipitation was carried out to detect the formation of the RUNX1-P62 complex. Autophagic flux was monitored by using RFP-GFP-LC3 tandem fluorescence reporters. For in vivo

SUBMITTER: Peng Y 

PROVIDER: S-EPMC12395785 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature