Hyperglycemia-Reduced Platelet-Derived Growth Factor-BB Expression Impairs Corneal Wound Healing in Diabetic Mice.

ABSTRACT: This study investigates the role and primary mechanisms of platelet-derived growth factor-BB (PDGF-BB) in regulating corneal epithelial injury repair and nerve regeneration under diabetic conditions. Type 1 diabetes was induced in C57BL/6 mice via streptozotocin injection. Platelet depletion was achieved using an anti-mouse GPIbα antibody. Platelet levels were quantified in intra- and extravascular compartments of the corneal limbus. Concentrations of growth factors released by activated platelets were measured in human and murine diabetic and nondiabetic samples. Diabetic mice with corneal epithelial wounds received PDGF-BB eye drops alone or combined with ERK1/2 or AKT inhibitors. Platelet-depleted mice were treated with PDGF-BB eye drops, while wild-type C57BL/6 mice received subconjunc

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12395822 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature