Engineered membraneless organelles in <i>Corynebacterium glutamicum</i> for enhanced indigoidine biosynthesis and antimicrobial peptide production.

ABSTRACT: Liquid-liquid phase separation (LLPS)-driven membraneless organelles (MLOs) have been employed to enhance metabolic efficiency in various microbial cell factories. However, their application in the industrial bacterium Corynebacterium glutamicum has not been explored. Here, we report the formation of liquid protein condensates in C. glutamicum using the RGG domain of Caenorhabditis elegans LAF-1. We optimized conditions for condensate formation, including the pre-induction period, inducer concentration, and cultivation temperature. Using the indigoidine biosynthesis pathway as a model, we demonstrated that LLPS-mediated MLOs enhanced indigoidine production. Furthermore, we applied these MLOs to modulate the toxicity of antimicrobial peptides (AMPs) to host cells, facil

SUBMITTER: Sun M 

PROVIDER: S-EPMC12395988 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature