Reverse transcription recombinase polymerase amplification-lateral flow assay for detection of pathogenic orthoflaviviruses in mosquito vectors.

ABSTRACT:

Background

The genus Orthoflavivirus primarily consists of arthropod-borne viruses capable of infecting vertebrate hosts and causing serious human diseases such as dengue fever, Zika fever, Japanese encephalitis, West Nile fever, and yellow fever. This study describes the development of a simple and field-deployable detection system for multiple pathogenic orthoflavivirus species using the recombinase polymerase amplification (RPA) technique.

Methods

Several previously published broad-specific primers targeting the genus Orthoflavivirus were evaluated. A new primer pair, FlaviPath-F and FlaviPath-R, was designed and tested for its applicability in an RPA assay. The RPA protocol was experimentally optimized, with a focus on determining the assay's sensitivity an

SUBMITTER: Thayanukul P 

PROVIDER: S-EPMC12396212 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature