Gene expression dynamics before and after zygotic gene activation in <i>Drosophila</i> early embryogenesis.

ABSTRACT: Post-transcriptional gene regulatory mechanisms are fundamental to the determination of gene expression dynamics and especially crucial for the earliest stages of animal development in which transcription is nearly silent. Here, we performed high-resolution total RNA sequencing and quantitative mass spectrometry analysis simultaneously on Drosophila maternal-to-zygotic transition (MZT). Further, this study is the first to report the proteome-wide quantitative changes in protein ubiquitination in Drosophila MZT. Our results indicate that timely ubiquitination of the distinct target proteins during MZT is essential for the downregulation of protein expression levels. Profiling of the RNA-associated proteome changes in Drosophila MZT suggested that RNA binding can be regulated w

SUBMITTER: Na Y 

PROVIDER: S-EPMC12396305 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature