Accurate MS-Based Diagnostic Amyloid Typing Using Endogenously Normalized Protein Intensities in Formalin-Fixed Paraffin-Embedded Tissue.

ABSTRACT: Amyloidoses are a group of diseases characterized by the pathological deposition of non-degradable misfolded protein fibrils. These include plasma cell neoplasms, chronic inflammatory conditions, and age-related disorders, among others. Precise identification of the fibril-forming, and thereby amyloidosis-type defining protein is crucial for prognosis and correct therapeutic intervention. While immunohistochemistry (IHC) is widely used for amyloid typing, it requires extensive interpretation expertise and can be limited by inconclusive staining results. Thus, mass spectrometry (MS), if available, has been proposed as the preferred method for amyloid typing by international specialized centers (United States and United Kingdom) using primarily spectral counts for quantification. Here, we in

SUBMITTER: Hollfoth V 

PROVIDER: S-EPMC12396419 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature