Profiles of HBV DNA integration in humans with hepatitis B virus infection: Insights for antiviral treatment.

ABSTRACT:

Background & aims

HBV integration profiles in the natural history of chronic HBV infection (CHB) have not been well-defined. Hence, we aimed to determine HBV integration profiles across different CHB phases.

Methods

We delineated integration profiles from liver biopsies of 55 patients in different CHB phases (3 HBsAg-positive/HBeAg-positive infection; 13 HBsAg-positive/HBeAg-positive hepatitis; 7 HBsAg-positive/HBeAg-negative infection; 12 HBsAg-positive/HBeAg-negative hepatitis; 10 HBsAg seroclearance; 10 occult HBV). Target-capture next-generation sequencing (NovaSeq-6000) was performed, and integrations were characterized on AVID (integrations defined as chimeric fusions in ≥1 soft-clipped reads and ≥2 total reads).

Results

HBV integrations were detected in 35 HBsA

SUBMITTER: Hui RW 

PROVIDER: S-EPMC12396433 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature