Escherichia coli FocA/B-dependent H⁺ and K⁺ fluxes: Influence of exogenous versus endogenous formate.

ABSTRACT: Escherichia coli translocates formate/formic acid bidirectionally across the cytoplasmic membrane by the FocA/FocB formate channels during fermentation. Depending on the pH and whether formate is supplied exogenously or generated internally, the mechanisms of translocation differ. This study elucidates the role of these channels in dependence on F_OF₁ ATPase activity in stationary phase cells after cultivation by mixed-carbon fermentation at pH 7.5. In cells cultivated with glucose plus glycerol, exogenously added formate increased the N,N'-dicyclohexylcarbodiimide (DCCD)-sensitive (F_OF₁ ATPase-dependent) proton flux in single or double foc mutants. Moreover, exogenously supplied formate also increased the DCCD-sensitive potassium flux, but only i

SUBMITTER: Grigoryan L 

PROVIDER: S-EPMC12396580 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature