PET imaging of TREM2 in amyloid-beta induced neuroinflammation.

ABSTRACT:

Purpose

The triggering receptor expressed on myeloid cells 2 (TREM2) has become a promising target for biologics in both monitoring and treating neuroinflammation in Alzheimer's disease (AD). This study aimed to develop and compare bispecific anti-TREM2 antibodies featuring different transferrin receptor (TfR) binders to enhance brain delivery, identifying the most suitable format for in vivo PET imaging of TREM2 in transgenic AD mice.

Methods

Three bispecific TREM2-antibody formats were produced and evaluated for their ability to cross the blood-brain barrier (BBB) via TfR-mediated transcytosis and bind TREM2. Blood concentration profiles up to 72 h post-injection (p.i.), and ex vivo brain uptake of iodine-125-labeled antibody constructs were quantified in App^NL-G-F

SUBMITTER: Dahlen AD 

PROVIDER: S-EPMC12396982 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature