Wedelolactone induces pyroptosis to suppress retinoblastoma by inhibiting the Nrf2/Keap1 signaling pathway.

ABSTRACT:

Background

Retinoblastoma is an intraocular malignancy with limited therapeutic options, imposing a severe health burden on young patients. Wedelolactone (WDL), a natural product from E. prostrata, possesses an anti-retinoblastoma activity, with the underlying regulatory mechanism remaining unknown.

Methods

Retinoblastoma cell lines and xenograft nude mouse models were treated with WDL and RTA-408, an agonist for nuclear factor-erythroid 2-related factor 2 (Nrf2). Western blotting was conducted to determine the protein expression levels of kelch-like ECH-associated protein 1 (Keap1) and Nrf2. We performed the cell counting kit-8 assay, the 5-ethynyl-2-deoxyuridine staining, and flow cytometry to detect cell viability, proliferation, and apoptosis, respectively. The tumor pro

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC12397164 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature