G-quadruplex-dependent transcriptional regulation by molecular condensation in the Bcl3 promoter.

ABSTRACT: G-quadruplexes (G4s) are pivotal in transcriptional regulation. Although the interaction between G4s and G4-binding transcription factors (TFs) is critical for G4-dependent transcriptional regulation, the detailed mechanism, especially TF enrichment at G4s and its correlation with transcriptional regulation, remains unknown. In this study, using specificity protein 1 (SP1) as a representative G4-binding TF, we examined the mechanism of G4-dependent transcriptional regulation. Genomic analysis revealed substantial enrichment of SP1 in the oncogenic Bcl3 promoter harboring G4-forming sequences. We demonstrated that the formation of transcriptional condensates and the transcriptional activation of the Bcl3 promoter are heavily dependent on G4-dependent SP1 binding. Moreover, dissociation of S

SUBMITTER: Yoo W 

PROVIDER: S-EPMC12397910 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature