Comprehensive bioinformatic analysis identifies potential therapeutic drugs for CryAB (R120G)-related cardiomyopathy.

ABSTRACT: CryAB, known as Alpha-B Crystallin, has also been shown to bind and increase the unfolding force of the filamentous protein. Mutation of CryAB at R120G causes serious cardiomyopathy but lacks ideal therapeutic drugs. In the present study, we constructed CryAB (R120G)-transgenic (TG) mice. One hundred and four differentially expressed genes (DEGs) in heart tissues of TG mice were screened through RNA sequencing. Gene ontology (GO) and KEGG pathway analyses highlighted enrichment in critical biological processes such as blood circulation regulation, contractile fiber formation, actin binding, thyroid hormone signaling, endocytosis, and alanine, aspartate, and glutamate metabolism. Protein-protein interaction (PPI) analysis pinpointed key hub genes, including DHTKD1, NKTR, SCN5A, CLCN1, RPA1,

SUBMITTER: Zheng J 

PROVIDER: S-EPMC12398125 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature