Diffuse reflectance and fluorescence spectroscopy for breast conserving surgery.

ABSTRACT:

Purpose

The major challenge in breast conserving surgery is the high rates of re-excision due to positive resection margins. This study evaluates whether a combined diffuse reflectance spectroscopy (DRS) and laser induced intrinsic fluorescence spectroscopy (IFS) technique can differentiate breast tissue sample types, towards the development of an intraoperative margin assessment tool.

Methods

Breast tissue samples were collected from patients undergoing breast cancer surgery. A handheld DRS-IFS probe was used on the frozen thawed ex-vivo  breast samples to acquire spectral data. Machine learning classifiers were used to determine sensitivity, specificity, overall diagnostic accuracy, and the area under the curve (AUC) against "gold-standard" histopathology ground truth.

SUBMITTER: Shanthakumar D 

PROVIDER: S-EPMC12398437 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature