Stage-specific requirement for METTL3-dependent m⁶A epitranscriptomic regulation during myogenesis.

ABSTRACT: The regulatory role of N⁶-methyladenosine (m⁶A) modification in skeletal muscle myogenesis and muscle homeostasis remains poorly characterized, particularly regarding the functional significance of methyltransferase-like 3 (METTL3), the catalytic subunit of the m⁶A methyltransferase complex (MTC), in myogenic regulation. Through systematic investigation of m⁶A epitranscriptomic remodeling during myogenesis, we demonstrate that METTL3-mediated m⁶As orchestrates myoblast fusion processes in both differentiation and regeneration contexts. Notably, we observed marked induction of Mettl3 expression post-injury, accompanied by substantial transcriptomic alterations in myogenesis-related pathways. High-resolution m⁶A mapping reveal

SUBMITTER: Tan YY 

PROVIDER: S-EPMC12398539 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature